Coding
INT5
Part:BBa_K3254006:Design
Designed by: Yeqing Zong Group: iGEM19_GENAS_China (2019-10-09)
Integrase Int5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 30
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 22
Illegal AgeI site found at 406
Illegal AgeI site found at 781 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was first reported in 2014. Two PstI site were eleminated by synonymous mutations.
Source
The original organism is the Streptomyces phage PhiK38-1. The DNA was coloned from the source plasmid(pCis2+7+8+5, see reference).
References
Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6.